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The Signaling and Pathogenesis of Staphylococci team, led by Tarek Msadek, a researcher in the Biology of Gram-positive Pathogens Unit at the Institut Pasteur (CNRS ERL 3526), is studying bacterial responses to environmental variations and their role in pathogenesis and host interactions.
In this study, we compared combinations of seven different endotoxin removal...
more Bacterial endotoxins have high immunogenicity. Phage biology studies as well as therapeutic phage applications necessitate highly purified phage particles.
At least one classic enterotoxin gene was present in 42.5% of the strains.
Interestingly, there were no differences in enterotoxin genes between S.
During the study of one of these systems, Wal KR, essential for bacterial survival, they characterized an additional component, Spd C, a membrane protein whose role was unknown.
This component interacts with the Wal KR system to control its activity and its absence leads to a strong decrease in virulence, biofilm formation (bacterial aggregates), and resistance to certain antibiotics.In this study, we compared combinations of seven different endotoxin removal strategies and validated their endotoxin removal efficacy for five different phages (i.e.four Pseudomonas aeruginosa phages and one Staphylococcus aureus phage).In one case, staphylococcus aureus methicillin sensitive was responsible for septic arthritis.In another case, SCJ infection was associated with pneumonitis.Fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) was used to visualize possible biofilm structures for a selection of middle ear effusion samples.34 MEE samples were collected from 21 patients of which 64.7 % were culture positive for bacteria and 47.0 % were culture positive for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aur... Phage biology studies as well as therapeutic phage applications necessitate highly purified phage particles.A research team has characterized a Staphylococcus aureus gene involved in virulence, biofilm formation and resistance to certain antibiotics.These results open up new avenues for understanding the control of S. is part of the natural skin flora, preferentially colonizing external mucosa in 30 to 50% of the population, healthy carriers who develop no symptoms.The most dangerous strains are those that display resistance to multiple antibiotics.This is the case of MRSA, resistant to Meticillin, widespread in hospitals and posing a major public health concern.